The correct operation and use of the centrifuge tube by the experimenter is very important for the accuracy of the experimental results. Therefore, in the experimental operation, it is necessary to master some methods and skills to use the centrifuge tube reasonably and improve the experimental efficiency.
The upper diameter of the centrifuge tube is round, and the lower end of the tube body is conical. The upper diameter is round to increase the mechanical strength, and the lower truncated cone can reduce its volume. When the micro-suspension is precipitated by centrifugation, the volume of the precipitate can be directly read, and the color and crystal shape can be easily observed. A microcentrifuge tube is a small centrifuge tube, also known as an EP (eppendorf) tube, which is used in conjunction with a microcentrifuge for the separation and centrifugation of micro-reagents.
Let's take a look at how to operate the centrifuge tube.
Centrifuge tube
How to use
1. Because the graduated centrifuge tube is a measuring device, it must be cleaned and dried before use.
2. When reading, be sure to observe the meniscus correctly, otherwise certain errors will be introduced.
3. When using, the number of centrifuge tubes should be determined according to the model of the centrifuge. If a centrifuge with double cannula is used for a single test, the same volume of liquid must be placed in the other centrifuge tube to maintain balance.
4. The centrifuge tube needs to be adapted to the centrifuge. According to the configuration of the centrifuge, use the appropriate length and thickness of centrifuge tubes.
5. After the centrifuge tube is put into the casing of the centrifuge for stratified separation, it should be stopped naturally when it stops rotating, and it must not be forced to stop by external force.
Notes
When using centrifuge tubes, do not use one tube for multiple times. Pay attention to the volatilization of samples and the leakage of some radioactive or corrosive samples; during the storage process, it is necessary to seal them well; to prevent the centrifuge tubes from being used during use Deformation occurs.
1. Do not put any substance on the centrifuge cover. After each use, be sure to clean the inner cavity and rotor.
2. If the high-speed microcentrifuge has not been used for a long time, the centrifuge cover should be opened for a period of time before use to dry the inner cavity.